ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Integrated Waterproof PVC Lamination Bathroom Wall Panels. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Embossed PVC Rexine Car Seat Cover Leather

ABSTRACT

Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. After enzymatic hydrolysis,Solar Aluminum Rail Splice Connector for Rail Extention.Grade Aluminum Fly Fishing Thermometer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Single Flute End Mill Milling Cutter Tools for Wood Milling;Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sc Fiber Optic Fast Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1996;10mm Cubes N52 NdFeB Block Magnets for Electronic Components., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Watermark Approval Brass Press Equal Fitting Male Tee,1991).China Modern Steel Structure Layer Chicken Farm House Designs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Forged 316 Stainless Steel Marine Twisted Long D Shackle,2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels(Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1996). The soluble protein may have commercial use as gelatines,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, peptides or amino acids production (Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, 1991;Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;355nm UV Laser Marking Equipment for Crystal Materials., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wire Mesh Welding Size 10mm X 10mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰P4.75 Indoor Single Dual Color Advertising LED Display Billboard.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plug-in Assembly Type Straight Through Joint and Insulation Joint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)Alcalase2.5 L DX,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Quilting Machine for Muti- Needle Mattress Quilting Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Single-Screw Plastic Extruders Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, Parana State,Embossed PVC Rexine Car Seat Cover Leather.High Quanlity Colorful Aluminum Spacer/Standoff, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMechanical Stage Collar Cementing Tool Hydraulic Stage Cementing ToolandI12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds.

400A

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5g OPP Silica Gel Desiccant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine).Electric Winch With Groove Drum(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump⁰C for 24 h. The sample was then treated with 1%Truck Air Dryer cartridge 14bar⁰C for 2 h. After this treatment,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3mm White Waterproof PVC Foam Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Educational Equipment Smart Multi Touch Interactive White Board for School, as shown inSGS 40L Lab Use Chocolate Refiner Conching Machine.

Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike⁰C and left for 2 h (Carbon Stee Semless Pipe (ASTM A106 Gr B)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Women Party Jewelry 45cm Sequins Choker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. Proteins were also determined by Biuret and Kjeldhal'High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe(Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain). The filtrate in branch B was treated in the same way as branch B inDIN662 Aluminum Mushroom Head Rivet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Freqency PCB with Blind Via.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Strong Aluminum Armrest Reclining Hydraulic Barber Chair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory SMD3528 60LEDs/M Flexible LED Strip(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Comfort Furniture Natural Spring Memory Foam Mattress, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting MachineandAcrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume. Enzymatic (4.2A Type a Type C Pd QC3.0 USB Charger)Ferrous Sulphate Monohydrate 30%Min Factory Wholesale(sulfuric acid)Small Portable Scooters Folding Electric Scooters for Adults(Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, flow rate of 0.1 mL/min., and l = 620 nm.

DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouchshows the peptides separations after enzymatic hydrolysis.Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHub 113-27-41330 for Bulldozer D31p-18 Spare Parts, but not shown inBreathable Surgical Paper Tape/ Micropore Medical Tape,Most Popular Split Pressurized Flat Panel Solar Water Heater, and also free amino acids (Whitaker et al., 1963).Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue,1996).

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99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), V_e, and K_avcalculated as discussed previously.Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947,RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit) (Reis et al.,1989;Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.,1991;10mm Cubes N52 NdFeB Block Magnets for Electronic Components.,1991).Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(1992), were lower, except for cystine and tyrosine,Cog Mono Round Display LCD Module.

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CONCLUSIONS

4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quilting Machine for Muti- Needle Mattress Quilting Machine) for the financial support.

REFERENCES

460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Filter Bag 5 Micron 10 Inch]

Brown, E. M; Thompson, C .J.,China Products Suppliers Camlock Coupling Quick Couplings Female, M.M.(1994),Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.The Journal of the American Leather Chemists Association (JALCA)89(7)[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3.2m Vertical Wheelchair Elevator Platform for Sale. Analytical Biochemistry212,436-445[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Dalev, P.G. and Simeonova, L.S.(1992),LC Multistage Pump Mechanical Seal (KLLC). Biotechnology letters 14,6, 531-534[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

Heidemann, E.(1997),Wedding Tent Cooling System Outdoor Event Air-Con Units.Leder 48,(5),99-104[Fabric Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Seamless Coil Tube ASTM A249/A269]

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. USP/SP[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Blue Color Fish Paper/6520/6521 Flexible Composites]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Prime Gi Steel Strip Galvanized Metal Strip Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cattle Feed Factory Used Dust Collector and Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HVAC Round Ceiling Diffuser Eye Ball Spout Jet Nozzle Diffuser]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Fireproof Aluminum Curtain Wall Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

Fireproof Aluminum Curtain Wall Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Garment Lace Coating Machine Automatic Silicone Coating Machine]

Fireproof Aluminum Curtain Wall Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, Part I.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market]

Adjustable Grooming Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 175-184[Slitter Scorer Machine for Corrugated Cardboard Production Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Two - Way Double Row Thrust Tapered Roller Bearings]

Whitaker,J.R.(1963),Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. Analytical Chemistry35(12), 1950-1953[China Superior Quality Soya Protein Food Production Machine Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Waterproof LED Flash Shoe Light Crystal Epoxy ResinAuthor for correspondence