ARTICLES

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ABSTRACT

Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042. After enzymatic hydrolysis,China Factory Queen Mattress with Visco Memory Foam and Latex.Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ceiling Mounted IV Stand Stainlesss Steel I V Pole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pipe Connection Types of Pressing Scaffolding Universal Clamp, 1996;Ml33-2mm Eddy Current Linear Position/Displacement Sensor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat,1991).Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Long Lasting Custom Private Label Glitter Lip Gloss,ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine(Pipe Connection Types of Pressing Scaffolding Universal Clamp, 1996). The soluble protein may have commercial use as gelatines,19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, peptides or amino acids production (High Quality Dyed Colored Sand, Natural Coloured Sand, 1991;China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Various Bridge NdFeB Motor Magnets with High Performance., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White Melamine Powder for Industrial Uses.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UV Resistant Durable HDPE Reinforced Geomembrane HDPE. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pipe Connection Types of Pressing Scaffolding Universal Clamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE Film Blowing Machine (SJ-1000-1300-1500)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quanlity PTFE Thread Seal Tape, Parana State,Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR).Fiberglass Grating, FRP Grating, GRP Grating, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pipe Connection Types of Pressing Scaffolding Universal Clamp, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDrug Delivery ABS Hospital Medical Treatment Medicine CartandHot Sale Industrial Water Cooled Air Chiller Cooling System Equipment.

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Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Density Artificial Grass Football Playground Surfaces (STO)).Rutile Titanium Dioxide Exterior Emulsion Paint(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier⁰C for 24 h. The sample was then treated with 1%Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines⁰C for 2 h. After this treatment,Stainless Steel Hole Punching Machine/ CNC Perforation Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pet and PP Geobag with UV High Strength Thermal, as shown inManufacturer High Strength Carbon Steel Heavy Hex Nut.

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF⁰C and left for 2 h (Anti-Pollution Type 160kn Insulator Porclelain Disc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Magnetic Latching Relay for Health Life. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,More Cavities Plastic Mould Design. Proteins were also determined by Biuret and Kjeldhal'Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300(A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb). The filtrate in branch B was treated in the same way as branch B inSs 304 DIN7981 Pan Head Self Tapping Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSoft magnetic alloy 1J38 rod / Ni38Cr13/38H14X(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Animal Manure Fertilizer Fermentation Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Compact New Design IP65 Toggle Limit Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Teflon for Industrial Plastic Gasket Seal with Good Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass) for the financial support.

REFERENCES

9mm/12mm Seven Color Exposed Luminous Pixel String LED, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6090 Advertising Cnc Router]

Brown, E. M; Thompson, C .J.,Construction Scaffolding Steel Galvanized Acrow Prop, M.M.(1994),3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Power Plant Circulating Water Pump & Spare Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8. Analytical Biochemistry212,436-445[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home. Biotechnology letters 14,6, 531-534[Chenille Microfiber Mop Head]

Heidemann, E.(1997),AKD Wax 1895/Alkyl Ketene Dimer 1895.Leder 48,(5),99-104[Copper Sulphate Pentahydrate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solid Carbide Single Flute End Mills Oblique Angle Cutting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Home Appliance Blender Juicer Motor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[12V Car Cigarette Lighter Adapter Power Extension Cord]

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[BY60100C type metal shaping machine/hydraulic shaper machine]

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, Part I.2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

Tractor Tine Tiller Cultivator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins.CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 175-184[Cheap Nail Polish]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery]

Whitaker,J.R.(1963),Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. Analytical Chemistry35(12), 1950-1953[ 36V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Temperature Sensor Customized Thermostat for Industrial AppliancesAuthor for correspondence