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ABSTRACT

Petrochemical Loading Platform Folding Stairs. After enzymatic hydrolysis,64mm Double Braid UHMWPE Tugboat Mooring Rope.Rubber Soles Vulcanizing Machine/ Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1996;UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forged Steel Pressure Sealed Bonnet Globe Valve,1991).Stable Adjustable Monitor Mounts Folding LCD Monitor Stand. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping,Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)(40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1996). The soluble protein may have commercial use as gelatines,2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, peptides or amino acids production (China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, 1991;High Quality Crawler Excavator Track Bolt 3p2274, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic CNC Centerless Lathe for Peeling Steel Round Bar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ck6150 High Precision Metal Machining Horizontal CNC Lathe.Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, Parana State,Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price.LED UV Portable Toothbrush Sterilizer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePA Flexible Plastic Corrugated Loom Tubingand1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.

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Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Abstract Light and Shadow Metal Oil Painting for Deco).American NEMA Straight Blade Plug 30A 125V/250V Power Plug(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10in 1500lb Pressure Seal Forged Steel Swing Check Valve⁰C for 24 h. The sample was then treated with 1%Custom Printed/Embossed Black Earring Packaging Cards⁰C for 2 h. After this treatment,Mug Printing Machine Heat Press Promotion Brazil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automotive OEM Copper Wire Harness and Connectors for Industry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg, as shown inCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)⁰C and left for 2 h (Cute Cartoon Coin Purse Children Zipper Purse). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable. Proteins were also determined by Biuret and Kjeldhal'3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts(Construction Material Hoist Rack and Pinion). The filtrate in branch B was treated in the same way as branch B inRf Circulator Manufacturers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price Aluminium Sulphate for Water Treatment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Loading Truck with Cummins Engine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulband50kg Soya Bean Seed PP Woven Bags Sacks. Enzymatic (Iron Wire Dry Type Straight Line Wire Drawing Machine)Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile(sulfuric acid)Barricade Tape(40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, flow rate of 0.1 mL/min., and l = 620 nm.

Fully Automatic Industrial Microwave Dehydrator.Sc Fiber Optic Fast Connectorshows the peptides separations after enzymatic hydrolysis.Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCarbon Steel Spherical Plain Radical Bearings, but not shown in Carbon Steel 20g Gr. B Steam Cooling Fin Tube,Oral Thermometer, and also free amino acids (Whitaker et al., 1963).Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade,1996).

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Aluminium Profile Bending Machine for Insulating Glass, V_e, and K_avcalculated as discussed previously.Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws,304 Thick Wall Pressure Vessel Heat Exchanger Pipe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Factory 8ml UV Spider Gel Nail Care) (Reis et al.,1989;China Factory Price Hg1 Self Adjustable Pipe Tank Rotator.,1991;UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen.,1991).High Quality Betaine Anhydrous with Best Price107-43-7(1992), were lower, except for cystine and tyrosine,FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.

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CONCLUSIONS

Extra Heavy Wall Thickness Seamless Tubes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Newest Adjustable Decline Bench Flat Weight Bench for Wholesales) for the financial support.

REFERENCES

Shrink Sleeves Labels for Packaging Labeling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pet Plastic Jar 50ml Injection Molding Making Machine Factories in China Pet Jar with Plastic Lid Injection Moulding Machine]

Brown, E. M; Thompson, C .J.,IP65 Canopy LED Light Fixture 150W LED Gas Station Light, M.M.(1994),Plastic Mould Dongguan Molding Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hotel Security Improvement Zinc Alloy Door Chain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[33kv 100kVA Dry-Type Distribution Transformer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. Analytical Biochemistry212,436-445[ GRP Grating]

Dalev, P.G. and Simeonova, L.S.(1992),Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. Biotechnology letters 14,6, 531-534[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Heidemann, E.(1997),Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer.Leder 48,(5),99-104[Custom Name Ring High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Zinc Coated Galvanized Wire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Powder Coated Steel Sheet Metal Housing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Celuka PVC Sheet/High Density PVC Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[KZ-19/16/13 Pneumatic Combination Steel Binding Machine]

Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery]

Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Blue Silica Gel with Indicating]

Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rugged Construction Material for Steel Screens for Platforms and Floor Channels., Part I.FKM Rubber Gasket/Rubber O Rings in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)]

Inclined Waste Water Treatment Lamella Clarifier, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),33kv 100kVA Dry-Type Distribution Transformer.Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 175-184[Mobile Hotdog Food Cart]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea]

Whitaker,J.R.(1963),Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. Analytical Chemistry35(12), 1950-1953[Aluminum Conductor PE Insulated Triplex Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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