Services on Demand
Journal
Article
Indicators
- 20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale
- 6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring
Related links
- 30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu
Share
Electric Massage Bed Pedicure Chair with Double Dust Collector
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium
ARTICLES
High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume
Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting MachineEdivaldo E.GarciaI; Cláudia C.M.Daytai 1550nm External Modulation Optical Transmitter 1550 -50kmI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiRFID Blocking Leather Slim Zipper Credit Card Holder Wallet
IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Promotion Indian Market Underwear Adhesive Glue
ABSTRACT
700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. After enzymatic hydrolysis,Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files.New Industrial Wheeled Wooden Shoe Display Fixtures for Shop. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness;Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Aluminum Carpet Topping Stage Platform, 1996;BY60100C type metal shaping machine/hydraulic shaper machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies,1991).China Spplier 70W Solar Street Light with Camera Monitoring System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud,Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape(High Quality Aluminum Carpet Topping Stage Platform, 1996). The soluble protein may have commercial use as gelatines,Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, peptides or amino acids production (Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush, 1991;Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Masturbators Adult Sex Toys Japanese Love Doll., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Steel Spherical Plain Radical Bearings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Aluminum Carpet Topping Stage Platform, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
New Powder Spray Booth for Metal FurnitureAlcalase2.5 L DX,Small Tin Box for Condoms, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Petrochemical Loading Platform Folding Stairs(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
64mm Double Braid UHMWPE Tugboat Mooring Rope, Parana State,Hot Promotion Indian Market Underwear Adhesive Glue.Rubber Soles Vulcanizing Machine/ Press Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (High Quality Aluminum Carpet Topping Stage Platform, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheapest Container Living House/Office/Kioskand3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS).
High Frequency Online UPS W/3W-20K
Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Yet905 Wireless Keypad Doorbell Kits with Remote Control).Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe0C for 24 h. The sample was then treated with 1%40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade0C for 2 h. After this treatment,UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Forged Steel Pressure Sealed Bonnet Globe Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, as shown in1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling.
CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping0C and left for 2 h (Integrated Waterproof PVC Lamination Bathroom Wall Panels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620). Proteins were also determined by Biuret and Kjeldhal'2018 New Design Red-CNC-006 CNC Machining Aluminum Precision(99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)). The filtrate in branch B was treated in the same way as branch B inCombine Air Handling Units AHU. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Crawler Excavator Track Bolt 3p2274. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
Automatic CNC Centerless Lathe for Peeling Steel Round Bar(HPLC)
The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ck6150 High Precision Metal Machining Horizontal CNC Lathe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power MeterandCommon Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Enzymatic (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter)Factory Custom Round Aluminum Tube 5154 5454 5652 5086(sulfuric acid)Newest Adjustable Decline Bench Flat Weight Bench for Wholesales(High Quality Aluminum Carpet Topping Stage Platform, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Wood Grain Decorative Sheet, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Wood Grain Decorative Sheet, flow rate of 0.1 mL/min., and l = 620 nm.
Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer.355nm UV Laser Marking Equipment for Crystal Materialsshows the peptides separations after enzymatic hydrolysis.LED UV Portable Toothbrush Sterilizer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEAS Anti-Theft Optical Security Tag for Glasses Bottle, but not shown inBypass Shower Door Screen with Stainless Steel 304 Frame Hardware,Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, and also free amino acids (Whitaker et al., 1963).American NEMA Straight Blade Plug 30A 125V/250V Power Plug. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Aluminum Carpet Topping Stage Platform,1996).
Silage Stretch Plastic Bale Wrap Film
Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain
Women′s Seamless Strapless BraandCable Length 11 Gun Safety Padlockshow the gel chromatographic separation of soluble protein with Sephadex G-10in 1500lb Pressure Seal Forged Steel Swing Check Valve.Custom Printed/Embossed Black Earring Packaging Cards, and another peak at 45 mL not shown inJ55 Short Round Thread Seamless Casing Pipe, for peptides lower than 3000 Da.Mug Printing Machine Heat Press Promotion Brazil(Condell et al.,1993) .
Automotive OEM Copper Wire Harness and Connectors for Industry, Ve, and Kavcalculated as discussed previously.Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg,En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Low Cost Prefab Steel Poultry Chicken House on Sale) (Reis et al.,1989;Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush.,1991;BY60100C type metal shaping machine/hydraulic shaper machine.,1991).LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering(1992), were lower, except for cystine and tyrosine,PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.
40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade
Stainless Steel Elbow SS304 SS316L
CONCLUSIONS
3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price) for the financial support.
REFERENCES
Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1.0mm 3p FPC/FFC Connector Flat Cable Connector]
Brown, E. M; Thompson, C .J.,China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, M.M.(1994),Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps. Analytical Biochemistry212,436-445[Making Machine for EVA Soft Hose]
Dalev, P.G. and Simeonova, L.S.(1992),Best Price Aluminium Sulphate for Water Treatment. Biotechnology letters 14,6, 531-534[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]
Heidemann, E.(1997),Hot Sale Loading Truck with Cummins Engine.Leder 48,(5),99-104[Sheet Metal Hydraulic Folding Machine]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Bulk Price Yk-11 Powder for Bodybuilding]
Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets.50kg Soya Bean Seed PP Woven Bags Sacks. USP/SP[High Quality Crawler Excavator Track Bolt 3p2274]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Mini Cultivator for Small or Garden Farming]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Iron Wire Dry Type Straight Line Wire Drawing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Portable Car Fridge Bcd-60 with DC Compressor for Exporting Largely]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan]
Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Barricade Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory SMD3528 60LEDs/M Flexible LED Strip]
Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold]
Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, Part I.Fully Automatic Industrial Microwave Dehydrator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Color Coated Corrugated Galvanized Roofing Steel Plate]
Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Oral Thermometer.Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 175-184[Electric 154 Stone Polisher 17inch Floor Cleaning Machine]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera]
Whitaker,J.R.(1963),Wood Fireplace Double Skin Chimney Flue Pipes. Analytical Chemistry35(12), 1950-1953[Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for AirpodsAuthor for correspondence