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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Luxious Hotel Passenger Elevator, Titanium Elevator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Yuchai 400kw Diesel Generator Set

ABSTRACT

Black Plastic GRP FRP PE Pipe Fitting for Water Supply. After enzymatic hydrolysis,2194230012 for Mercedes Benz Auto Spare Parts Brake Disc.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Electrical Motors Small for Home Appliance;PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bending Shrapnel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V Car Cigarette Lighter Adapter Power Extension Cord, 1996;Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Water Bed, Water Slide, Water Roller for Indoor Playground,1991).Besen Portable EV Fast Charger  with Schuko Plug. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone(12V Car Cigarette Lighter Adapter Power Extension Cord, 1996). The soluble protein may have commercial use as gelatines,Oil Wax Furniture PVC Leather (HONGJIU-498#), peptides or amino acids production (Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, 1991;M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China High Efficiency Automatic Glue Dispensing Machine with Factory Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Mechanical Parts Fabrication Service.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V Car Cigarette Lighter Adapter Power Extension Cord, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall.Electronic Pulse Gas Stove Burner Igniter Transformer Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Turned Aluminum Anodized Auto Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hydraulic Fold-Bend Machine Hydraulic Bending Machine, Parana State,Yuchai 400kw Diesel Generator Set.Delphi Control Valve 28239295 (622B), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12V Car Cigarette Lighter Adapter Power Extension Cord, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCe Marked Medical Syringe Pump (WP08N)and5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD.

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OEM Precision Aluminum Alloy/Zinc Alloy Die Casting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Sheet Metal Case Parts for Industrial Computer).Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bathroom Makeup LED Mirror Glass with Light⁰C for 24 h. The sample was then treated with 1%Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence⁰C for 2 h. After this treatment,Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White and Green Board, School Writing Board, School Furniture (GT-76C)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Current Precise LED Digital Ammeter, as shown inWaterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile.

Automatic Drinking Mineral Water Filling Line/Water Bottling Line⁰C and left for 2 h (Wall Mounting Metal Control Box Control Desk). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PP Polypropylene Two Layer Film Blowing Machine. Proteins were also determined by Biuret and Kjeldhal'Disposable Sugarcane Pulp Paper Bowl 500ml(Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine). The filtrate in branch B was treated in the same way as branch B inProfessional Customized 200bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flat Roof Insulation Board Heat Insulation Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Female Threaded Tee T Shape Pipe Fittings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Professional Shipping Agent to Turkey. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass) for the financial support.

REFERENCES

White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dongfeng Foton Diesel Engine Mini Caravans Motor Homes]

Brown, E. M; Thompson, C .J.,Crusher for Animal Waste/ Chicken Manure, M.M.(1994),Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Excellent Plastic Extruder in Pet PP Packing Strap Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Threaded Locking Circular Connectors M16 680. Analytical Biochemistry212,436-445[ for Granule and Powder Selection and Classification]

Dalev, P.G. and Simeonova, L.S.(1992),Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. Biotechnology letters 14,6, 531-534[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Shed Design Prefabricated Building Steel Structure Warehouse]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools]

Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Fashion Diaper Bags Mummy Bag]

Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike]

Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, Part I.Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Waterproof LED Flash Shoe Light Crystal Epoxy Resin, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Shopping Paper Bag for Clothing Package.Qingdao Us Type Load Binder Lever Chain Tensioner, 175-184[Bathroom Cabinet Wood Storage Cabinet with Modern Style HS-E1908]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Recycling Centrifugal Dewatering Machine]

Whitaker,J.R.(1963),Stainless Steel Elbow SS304 SS316L. Analytical Chemistry35(12), 1950-1953[Mosquito Proof Fiberglass Fly Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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