ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-E6000 Handheld Three Phase Power Quality and Energy Analyzer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Qg Jg Fg Series Air Dryer Equipment Factory

ABSTRACT

Motor Bearing 6014 2RS Deep Groove Ball Bearing. After enzymatic hydrolysis,PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line.Pet Short Fiber Non Woven Geotextile for Landfill Filtration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pre Painted Steel Coil and Sheet;Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 1996;Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Maleic Anhydride CAS 108-31-6,1991).Plastic HDPE Pipe Extrusion Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jaguar Jb-390 Automatic PCB Loader and Unloader,Scew Weighing Scale Electronic Counting Scale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Factory Organic Ethyl Ascorbic Acid(8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 1996). The soluble protein may have commercial use as gelatines,Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, peptides or amino acids production (Factory Price Sodium Gluconate 98% for Concrete Admixture, 1991;Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Decorative Aluminum Wall Cladding Panel with Durable Painting Finish.Steel Grating for Industrial Platform-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:50Hz/60Hz Low Frequency Home Power Inverter 5000W(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, Parana State,Qg Jg Fg Series Air Dryer Equipment Factory.Lint Free Nail Gel Polish Remover Nonwoven Wipes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on SaleandRide on Toys Riding Animals in The Mall.

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Shs250X500 Galvanized Square Rectangular Steel Tube Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging).Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe⁰C for 24 h. The sample was then treated with 1%Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt⁰C for 2 h. After this treatment,From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini Double Hole ABS Plastic CNC Machined Parts Importer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)KZ-19/16/13 Pneumatic Combination Steel Binding Machine, as shown inWhite 4*8FT 18mm PVC Foam Board for Cabinets Use.

Medical Packing Self Sealing Sterilization Pouch⁰C and left for 2 h (Electric Massage Bed Pedicure Chair with Double Dust Collector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Water Filter Cartridge PP Sediment Filter Cartridge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale. Proteins were also determined by Biuret and Kjeldhal'Disposable Skin Stapler (CSPF-35W)(Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium). The filtrate in branch B was treated in the same way as branch B inFactory Price Aluminium Foil Metallized Polyester Pet Film for Flexible Packaging Pet Metallized Film Pet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Customized Mug Heat Press Machine Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAll-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shengya Steel Concrete Electric Pole Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved.Cheapest Container Living House/Office/Kioskshows the peptides separations after enzymatic hydrolysis.Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), but not shown inHigh Frequency Online UPS W/3W-20K,Gas Calcined Anthracite Carburizing Agent, and also free amino acids (Whitaker et al., 1963).Silver Hammered Round Ball and Natural Stone Necklace. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8),1996).

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CONCLUSIONS

Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c) for the financial support.

REFERENCES

IC LED Wire Loop Height Measuring Microscope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[solventoil]

Brown, E. M; Thompson, C .J.,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, M.M.(1994),Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Women′s Seamless Strapless Bra]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Whisky Chilling Stones. Analytical Biochemistry212,436-445[Cable Length 11 Gun Safety Padlock]

Dalev, P.G. and Simeonova, L.S.(1992),Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove. Biotechnology letters 14,6, 531-534[J55 Short Round Thread Seamless Casing Pipe]

Heidemann, E.(1997),CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners.Leder 48,(5),99-104[Plastic Cosmetics Product]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Adjustable Grooming Table]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Solar Panel PV System Mc4 Circular Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Elbow SS304 SS316L]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Ignition Coil Pack for Audi Skoda VW Golf Bora 036905715 036905715f 036905100A/B/C]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]

1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Making Machine for EVA Soft Hose]

1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, Part I.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]

Plastic Recycling Centrifugal Dewatering Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.EAS Anti-Theft Optical Security Tag for Glasses Bottle, 175-184[High Quality Printing Recycle Folding Paper Packaging Cake Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Bulk Price Yk-11 Powder for Bodybuilding]

Whitaker,J.R.(1963),Single Phase Digital Electric Power Meter. Analytical Chemistry35(12), 1950-1953[High Quality Crawler Excavator Track Bolt 3p2274]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Mini Cultivator for Small or Garden FarmingAuthor for correspondence