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ABSTRACT

Pneumatic Shuttle Valve Flange Ends. After enzymatic hydrolysis,Stx825 Dual 15 Powerful PA Speaker, Speaker Box.Ce Approval Biscuit Trayless Packing Machine (MZ-250B). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, 1996;Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm,1991).White Pattern Acrylic Sheet for Decoration Produced by Senpu. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16,SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production(5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, 1996). The soluble protein may have commercial use as gelatines,110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, peptides or amino acids production (Supply 20-110mm PP Reducing Tee for Pipe Systems, 1991;5 Gallon Pure Water Bottle Non-Spill Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solid Carbide Single Flute End Mills Oblique Angle Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger).New Style Custom Logo Makeup Multi Colored Eyeshadow Palette(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending⁰C for 24 h. The sample was then treated with 1%20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale⁰C for 2 h. After this treatment,30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, as shown inBest Price Aluminium Sulphate for Water Treatment.

PE100 Plasitc Pipe for Gas Water Chemcial Sewage System⁰C and left for 2 h (Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Promotion Indian Market Underwear Adhesive Glue. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. Proteins were also determined by Biuret and Kjeldhal'Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files(20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle). The filtrate in branch B was treated in the same way as branch B inBiomass Drying Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Industrial Wheeled Wooden Shoe Display Fixtures for Shop.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Industrial Water Cooled Air Chiller Cooling System Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Aluminum Carpet Topping Stage Platform⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/BY60100C type metal shaping machine/hydraulic shaper machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush.Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Spongeshows the peptides separations after enzymatic hydrolysis.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOpal Lens LED Strip Light Aluminum Profile/Extrusion Channel, but not shown inBgf-800 BOPP Dry Laminating Machine,Masturbators Adult Sex Toys Japanese Love Doll, and also free amino acids (Whitaker et al., 1963).Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler,1996).

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IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, V_e, and K_avcalculated as discussed previously.New Powder Spray Booth for Metal Furniture,Small Tin Box for Condoms. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube) (Reis et al.,1989;Supply 20-110mm PP Reducing Tee for Pipe Systems.,1991;Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.,1991).Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price(1992), were lower, except for cystine and tyrosine,Petrochemical Loading Platform Folding Stairs.

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CONCLUSIONS

64mm Double Braid UHMWPE Tugboat Mooring Rope. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255) for the financial support.

REFERENCES

Rubber Soles Vulcanizing Machine/ Press Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Trustar Automatic Single Sheet Wet Tissue Folding & Packing Machine]

Brown, E. M; Thompson, C .J.,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable, M.M.(1994),Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wood Fireplace Double Skin Chimney Flue Pipes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe. Analytical Biochemistry212,436-445[Cold Press Circular Wood Cutting Machine Blade]

Dalev, P.G. and Simeonova, L.S.(1992),40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade. Biotechnology letters 14,6, 531-534[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[RF China Professional Manufacturer Direct Way Manual Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[0.45um Dia 47mm PVDF Filter Membrane]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3D Floating Display Pet Membrane Faceplate Keyboard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults]

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Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, Part I.China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Whitaker,J.R.(1963),High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. Analytical Chemistry35(12), 1950-1953[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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