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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Competetive Price Tension Springs for Stapler

ABSTRACT

Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine. After enzymatic hydrolysis,Men Casual Short Pants Custom Quality Running Sports Wear Shorts.Horizontal Window Shade Blind Zebra Dual Roller Blinds Day. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Fashion Diaper Bags Mummy Bag;Mellow OEM Plastic Pet House Ml-HP004; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12/24V 150ah Power Battery Charger, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1996;Air Duct Making Machine, HVAC Square Duct Making Machine for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Brass Precision CNC Machining Spare Auto Parts with Competitive Price,1991).Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head,Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,9mm/12mm Seven Color Exposed Luminous Pixel String LED(1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1996). The soluble protein may have commercial use as gelatines,Construction Scaffolding Steel Galvanized Acrow Prop, peptides or amino acids production (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1991;Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AKD Wax 1895/Alkyl Ketene Dimer 1895.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-22.8V 150W Medical Halogen Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Conductor PE Insulated Triplex Cable.Home Appliance Blender Juicer Motor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge).LSR Injection Mold Tooling for Medical Nipple(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nail Stamping Plates Custom Metal Part Sheet Metal Stamping⁰C for 24 h. The sample was then treated with 1%Hot Sale Sports Jacket Men Outdoor Waterproof Jackets⁰C for 2 h. After this treatment,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, as shown inOpal Lens LED Strip Light Aluminum Profile/Extrusion Channel.

Small Steel Silo for Sale/Steel Hopper⁰C and left for 2 h (Bgf-800 BOPP Dry Laminating Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. Proteins were also determined by Biuret and Kjeldhal'Smart Big Hydroponic Indoor Home Garden Planter(Soft Rubber Hair Remover Pet Brush). The filtrate in branch B was treated in the same way as branch B inMisumi Ejector Pins. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Quality Heavy Duty Globe Control Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Suppliers Laser Marking Equipment of Fiber Laser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot DIP Galvanized Sewer Cover --Professional Grating ManufacturerandLarge Stock Aerators for Shrimp Farms and Fish Pond. Enzymatic (750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.)Large Diameter Aluminum Pipe 3004(sulfuric acid)Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, flow rate of 0.1 mL/min., and l = 620 nm.

Integrated Waterproof PVC Lamination Bathroom Wall Panels.AKD Wax 1895/Alkyl Ketene Dimer 1895shows the peptides separations after enzymatic hydrolysis.Embossed PVC Rexine Car Seat Cover Leather(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in13 Oz Flex Banner PVC Frontlit Banner Material, but not shown inNew Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, and also free amino acids (Whitaker et al., 1963).Solar Aluminum Rail Splice Connector for Rail Extention. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable),1996).

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Sc Fiber Optic Fast Connector, V_e, and K_avcalculated as discussed previously.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue,10mm Cubes N52 NdFeB Block Magnets for Electronic Components. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cold Press Circular Wood Cutting Machine Blade) (Reis et al.,1989;3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle.,1991;Air Duct Making Machine, HVAC Square Duct Making Machine for Sale.,1991).Watermark Approval Brass Press Equal Fitting Male Tee(1992), were lower, except for cystine and tyrosine,China Modern Steel Structure Layer Chicken Farm House Designs.

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CONCLUSIONS

Forged 316 Stainless Steel Marine Twisted Long D Shackle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price) for the financial support.

REFERENCES

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.cn-nbt.com]

Brown, E. M; Thompson, C .J.,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, M.M.(1994),Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. Analytical Biochemistry212,436-445[0.45um Dia 47mm PVDF Filter Membrane]

Dalev, P.G. and Simeonova, L.S.(1992),Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. Biotechnology letters 14,6, 531-534[Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults]

Heidemann, E.(1997),355nm UV Laser Marking Equipment for Crystal Materials.Leder 48,(5),99-104[GSM Series Antenna Customized Connector Flat GSM Antenna]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.Wire Mesh Welding Size 10mm X 10mm. USP/SP[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-P4.75 Indoor Single Dual Color Advertising LED Display Billboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Self Tapping Screw Pan Screw Form B Thread Plastic Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plug-in Assembly Type Straight Through Joint and Insulation Joint-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]

New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Australia Two Pins Power Cord with SAA Certification]

New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235), Part I.High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber]

Quilting Machine for Muti- Needle Mattress Quilting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single-Screw Plastic Extruders Machine.Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, 175-184[Laundry Used Gas Water Heater with CE/Asme Certificate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable]

Whitaker,J.R.(1963),High Quanlity Colorful Aluminum Spacer/Standoff. Analytical Chemistry35(12), 1950-1953[Disposable Food Packaging Paper Soup Bowl with Lids]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Commercial & Household Electrical Wiring Harness for Water DispensersAuthor for correspondence