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ABSTRACT

New Fashion Diaper Bags Mummy Bag. After enzymatic hydrolysis,Mellow OEM Plastic Pet House Ml-HP004.12/24V 150ah Power Battery Charger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable);Air Duct Making Machine, HVAC Square Duct Making Machine for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Brass Precision CNC Machining Spare Auto Parts with Competitive Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, 1996;044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t,1991).9mm/12mm Seven Color Exposed Luminous Pixel String LED. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Construction Scaffolding Steel Galvanized Acrow Prop,3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8(Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, 1996). The soluble protein may have commercial use as gelatines,Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home, peptides or amino acids production (AKD Wax 1895/Alkyl Ketene Dimer 1895, 1991;22.8V 150W Medical Halogen Lamp, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Conductor PE Insulated Triplex Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Home Appliance Blender Juicer Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Smart Big Hydroponic Indoor Home Garden Planter⁰C and left for 2 h (Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Quality Heavy Duty Globe Control Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. Proteins were also determined by Biuret and Kjeldhal'China Suppliers Laser Marking Equipment of Fiber Laser(Factory SMD3528 60LEDs/M Flexible LED Strip). The filtrate in branch B was treated in the same way as branch B in24 Core Fiber Optic Cable Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bonfiglioli Ta Series Helical Gearing Gearbox.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Stock Aerators for Shrimp Farms and Fish Pond⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle., pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Diameter Aluminum Pipe 3004, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle) for the financial support.

REFERENCES

Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Crackle Glass Beads]

Brown, E. M; Thompson, C .J.,Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, M.M.(1994),355nm UV Laser Marking Equipment for Crystal Materials.The Journal of the American Leather Chemists Association (JALCA)89(7)[Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. Analytical Biochemistry212,436-445[Full-Automatic Folding Napkin Paper Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Wire Mesh Welding Size 10mm X 10mm. Biotechnology letters 14,6, 531-534[Magnetic Latching Relay for Health Life]

Heidemann, E.(1997),P4.75 Indoor Single Dual Color Advertising LED Display Billboard.Leder 48,(5),99-104[Car Parking Lifter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Soda Ash Dense]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 45W 50W Commercial Recessed COB LED Downlight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[125khz Tk4100 Rfid Card Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quilting Machine for Muti- Needle Mattress Quilting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Carbide Mould Parts Moulding Parts]

High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cog Mono Round Display LCD Module]

High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Single-Screw Plastic Extruders Machine, Part I.Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

High Quanlity Colorful Aluminum Spacer/Standoff, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5g OPP Silica Gel Desiccant.Electric Winch With Groove Drum, 175-184[Piston Rod For Hydraulic Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Quality Heavy Duty Globe Control Valve]

Whitaker,J.R.(1963),Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. Analytical Chemistry35(12), 1950-1953[Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sany 20ton Mobile Crane Stc200 100 Ton Mobile CraneAuthor for correspondence