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ABSTRACT

Qg Jg Fg Series Air Dryer Equipment Factory. After enzymatic hydrolysis,Motor Bearing 6014 2RS Deep Groove Ball Bearing.PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pet Short Fiber Non Woven Geotextile for Landfill Filtration;Pre Painted Steel Coil and Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, 1996;8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion,1991).Maleic Anhydride CAS 108-31-6. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic HDPE Pipe Extrusion Making Machine,Jaguar Jb-390 Automatic PCB Loader and Unloader,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Scew Weighing Scale Electronic Counting Scale(Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, 1996). The soluble protein may have commercial use as gelatines,China Factory Organic Ethyl Ascorbic Acid, peptides or amino acids production (Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, 1991;Factory Price Sodium Gluconate 98% for Concrete Admixture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pneumatic Cut off Ball Valve with Flange Thread Weld End., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.Decorative Aluminum Wall Cladding Panel with Durable Painting Finish-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Grating for Industrial Platform(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Lint Free Nail Gel Polish Remover Nonwoven Wipes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Promotion Indian Market Underwear Adhesive Glue).Shs250X500 Galvanized Square Rectangular Steel Tube Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool⁰C for 24 h. The sample was then treated with 1%Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe⁰C for 2 h. After this treatment,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Double Hole ABS Plastic CNC Machined Parts Importer, as shown in3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube.

KZ-19/16/13 Pneumatic Combination Steel Binding Machine⁰C and left for 2 h (1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Packing Self Sealing Sterilization Pouch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Water Filter Cartridge PP Sediment Filter Cartridge. Proteins were also determined by Biuret and Kjeldhal'Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale(Wind Protection Screen. Wind Dust Netting). The filtrate in branch B was treated in the same way as branch B inMechanical Lug Manufacturers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Disposable Skin Stapler (CSPF-35W).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Customized Mug Heat Press Machine Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shengya Steel Concrete Electric Pole Mould, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Auto Spare Parts Ignition Coil 22448-2y001andQuick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable. Enzymatic (Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles)China Manufacturer Garbage Incinerator(sulfuric acid)China water meter factory water flow meter vane wheel(Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4 Heads High Speed Professional Paper Cardboard Laser Cutter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4 Heads High Speed Professional Paper Cardboard Laser Cutter, flow rate of 0.1 mL/min., and l = 620 nm.

Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll.Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approvedshows the peptides separations after enzymatic hydrolysis.Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, but not shown inBubule 17 Inch PP Carry on Kids Travel Luggage for Children,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, and also free amino acids (Whitaker et al., 1963).Gas Calcined Anthracite Carburizing Agent. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor,1996).

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12 Teeth Green Painting Steel Rake, V_e, and K_avcalculated as discussed previously.Car Parking or Access Control 2.4 GHz Active RFID Card,Screw Press Dewatering Machine for Handling The Reject From Paper Mill. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Document Storage Durable Metal Wire Mesh Decking for Pallet Rack) (Reis et al.,1989;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details.,1991;8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8).,1991).C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale(1992), were lower, except for cystine and tyrosine,4lz-4.5 Small Tank Kubota Similar Rice Harvester.

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CONCLUSIONS

High Quality Automatic Snack Food Lab Twin Screw Extruder Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure) for the financial support.

REFERENCES

Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Leather Choker Necklace]

Brown, E. M; Thompson, C .J.,IC LED Wire Loop Height Measuring Microscope, M.M.(1994),Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Trench Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A. Analytical Biochemistry212,436-445[Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Whisky Chilling Stones. Biotechnology letters 14,6, 531-534[Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46]

Heidemann, E.(1997),Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove.Leder 48,(5),99-104[Good Quality and Long Time Warranty Hand Held Gold Metal Detector Manufacture Used for Gold Silver Treasure Searching and Sourcing Black and Orange Color]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[13.8cm Reed Diffuser Clear Glass Bottle for Aroma]

2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners.Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. USP/SP[Dry & Wet Concrete Surface Metal Grinding Wing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Quality Custom Car Power Window Cable for Ford Car: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Antifreeze Pump Retarder Sodium Naphthalene Sulphonate Formaldehyde]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool]

Solar Panel PV System Mc4 Circular Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

Solar Panel PV System Mc4 Circular Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 400A]

Solar Panel PV System Mc4 Circular Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, Part I.Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Recycling Centrifugal Dewatering Machine.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, 175-184[fishermen s hat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SGS 40L Lab Use Chocolate Refiner Conching Machine]

Whitaker,J.R.(1963),EAS Anti-Theft Optical Security Tag for Glasses Bottle. Analytical Chemistry35(12), 1950-1953[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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