ARTICLES

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ABSTRACT

High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder. After enzymatic hydrolysis,RCA Audio Connector Male Gold-Plated Cable Jack Plug Male.Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home Design Solid Wood Double Adult Bed Bedroom Furniture, 1996;Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns,1991).304 Precision Professional Dimpled Stainless Steel Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence,Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling(Home Design Solid Wood Double Adult Bed Bedroom Furniture, 1996). The soluble protein may have commercial use as gelatines,Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, peptides or amino acids production (Special Shape Spring with Good Quality and Fast Delivery, 1991;Better Quality White Coated Fbb Paper Ivory Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Excellent Plastic Extruder in Pet PP Packing Strap Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home Design Solid Wood Double Adult Bed Bedroom Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200.2019 New Advertising Inflatable LED Jellyfish Balloon for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tree Root Grinder Stump Grinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment, Parana State,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set.Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Home Design Solid Wood Double Adult Bed Bedroom Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDocument Storage Durable Metal Wire Mesh Decking for Pallet RackandHot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes.

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Programmable Salt Spray Test Chamber for Corrosion Testing/Test(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Galvanized Trench Cover).Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FKM Bearing Oil Seal /Customized⁰C for 24 h. The sample was then treated with 1%Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes⁰C for 2 h. After this treatment,T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Max Twintier Rb441t Using Wire Coils Tw1061t, as shown inDust Hood Fume Extraction Systems for Robotic Laser Cutting.

Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm⁰C and left for 2 h (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer Supply Durable Grey Color Non-Slip EVA Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Proteins were also determined by Biuret and Kjeldhal'Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box(Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46). The filtrate in branch B was treated in the same way as branch B inStorage Silo Tanks for Livestock Feed/Grain Silo Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Captain Woodwork Sanding Machine/Sanding Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAtg39 100W LED Flood Light Wall Light Wall Washer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gold Inlaid 24K Gold / Flowers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

13.8cm Reed Diffuser Clear Glass Bottle for AromaandYashi 65inch Infrared Touch Whiteboard Interactive Smart Board. Enzymatic (Hydraulic Cutter Bread Machine (ZMF-20Y))9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board(sulfuric acid)Yet905 Wireless Keypad Doorbell Kits with Remote Control(Home Design Solid Wood Double Adult Bed Bedroom Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), flow rate of 0.1 mL/min., and l = 620 nm.

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I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbudsand 400Ashow the gel chromatographic separation of soluble protein with Sephadex G-HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.Ceiling Mounted IV Stand Stainlesss Steel I V Pole, and another peak at 45 mL not shown inAutomatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, for peptides lower than 3000 Da.Pipe Connection Types of Pressing Scaffolding Universal Clamp(Condell et al.,1993) .

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Ml33-2mm Eddy Current Linear Position/Displacement Sensor, V_e, and K_avcalculated as discussed previously.Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Carbon Stee Semless Pipe (ASTM A106 Gr B)) (Reis et al.,1989;Special Shape Spring with Good Quality and Fast Delivery.,1991;Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont.,1991).Wholesale Long Lasting Custom Private Label Glitter Lip Gloss(1992), were lower, except for cystine and tyrosine,ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable.

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CONCLUSIONS

10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine) for the financial support.

REFERENCES

19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Roto Molded Camping Cooler]

Brown, E. M; Thompson, C .J.,High Quality Dyed Colored Sand, Natural Coloured Sand, M.M.(1994),China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Various Bridge NdFeB Motor Magnets with High Performance. Analytical Biochemistry212,436-445[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Dalev, P.G. and Simeonova, L.S.(1992),White Melamine Powder for Industrial Uses. Biotechnology letters 14,6, 531-534[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Heidemann, E.(1997),UV Resistant Durable HDPE Reinforced Geomembrane HDPE.Leder 48,(5),99-104[ASME/ANSI B16.9 Stainless Steel Sch40/80 Butt Welding Reducing Tee]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch. USP/SP[ Swine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PE Film Blowing Machine (SJ-1000-1300-1500): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Monk Strap Oxford Loafer Driving Formal Dress Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),304 304L 904L 309S 310S Stainless Steel Design Checkered Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass, Part I.2017 Factory Directly Oversized Women Sunglasses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

High Quanlity PTFE Thread Seal Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fiberglass Grating, FRP Grating, GRP Grating.Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium, 175-184[Double Injection Mold]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

Whitaker,J.R.(1963),Rutile Titanium Dioxide Exterior Emulsion Paint. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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