ARTICLES

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ABSTRACT

Car Parking or Access Control 2.4 GHz Active RFID Card. After enzymatic hydrolysis,Screw Press Dewatering Machine for Handling The Reject From Paper Mill.C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IC LED Wire Loop Height Measuring Microscope, 1996;Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A,1991).Stainless Steel Whisky Chilling Stones. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing(IC LED Wire Loop Height Measuring Microscope, 1996). The soluble protein may have commercial use as gelatines,Good Quality Custom Car Power Window Cable for Ford Car, peptides or amino acids production (Solar Panel PV System Mc4 Circular Connector, 1991;1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IC LED Wire Loop Height Measuring Microscope, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Recycling Centrifugal Dewatering Machine.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:EAS Anti-Theft Optical Security Tag for Glasses Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Glass Disc Suspension Insulators).Nonwovenface Mask Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Professional Indoor&Outdoor Trampoline Park⁰C for 24 h. The sample was then treated with 1%Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10⁰C for 2 h. After this treatment,HVAC Ventilation Supply Air Return Grille Air Vent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard, as shown inRCA Audio Connector Male Gold-Plated Cable Jack Plug Male.

High Output Vulcanizing Press Machine⁰C and left for 2 h (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. Proteins were also determined by Biuret and Kjeldhal'Transmission Ultrasound Gel /20ml, 250ml, 5L(30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu). The filtrate in branch B was treated in the same way as branch B inFestival Event Safety Access Control Customizable Woven Fabric Cloth RFID Wristband. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade Ivory Paper Cupcake Boxes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 New Style High Quality Enamel Bowl with Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Roots Vacuum, Vacuum Pump, Pulp Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Non-Ionic Polyacrylamide FlocculantandCarbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. Enzymatic (Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads)Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker(sulfuric acid)IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera(IC LED Wire Loop Height Measuring Microscope, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%European Hotel Decorative Natual Shell Square Standing Floor Lamp, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%European Hotel Decorative Natual Shell Square Standing Floor Lamp, flow rate of 0.1 mL/min., and l = 620 nm.

Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT.Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machineshows the peptides separations after enzymatic hydrolysis.Pengkai Domestic Underground Sewage Treatment Plant Aao Process(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, but not shown inOutdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair,PV Serial Bevel Gearbox for Drilling Machine, and also free amino acids (Whitaker et al., 1963).Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IC LED Wire Loop Height Measuring Microscope,1996).

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CONCLUSIONS

Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2W 100lm LED Solar Lawn Light for Garden or Yard Lighting) for the financial support.

REFERENCES

Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Cost Galvanized Prefab Steel Structure Warehouse Storage Shed]

Brown, E. M; Thompson, C .J.,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, M.M.(1994),Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays. Analytical Biochemistry212,436-445[Private Label OEM Best Cute Waterproof Lip Pluming Gloss]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. Biotechnology letters 14,6, 531-534[More Cavities Plastic Mould Design]

Heidemann, E.(1997),Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.Leder 48,(5),99-104[Imperial Roller Bearings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yuchai 400kw Diesel Generator Set]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chinese Color Steel Glazed Tile Purlin Roll Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.mybarberchairs.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest]

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, Part I.ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rectangular Lunch Tin Box with Handle]

Whitaker,J.R.(1963),UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. Analytical Chemistry35(12), 1950-1953[Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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