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ABSTRACT

OEM Round Stainless Steel Fruit Plate Tray. After enzymatic hydrolysis,Factory Supply Industry Grade Sodium Gluconate.Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, 1996;Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Conventional Best-Selling Household Bamboo Craft Chopsticks,1991).High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Marked Medical Syringe Pump (WP08N),New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet(Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, 1996). The soluble protein may have commercial use as gelatines,CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera, peptides or amino acids production (Electro Galvanized Low Carbon Straight Cut Wire, 1991;Geotextile for Roofs, Civil Applications. Railway Work, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pneumatic Shuttle Valve Flange Ends., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stx825 Dual 15 Powerful PA Speaker, Speaker Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Approval Biscuit Trayless Packing Machine (MZ-250B). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Different Size of Ring NdFeB Magnet).110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Supply 20-110mm PP Reducing Tee for Pipe Systems⁰C for 24 h. The sample was then treated with 1%5 Gallon Pure Water Bottle Non-Spill Cap⁰C for 2 h. After this treatment,Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solid Carbide Single Flute End Mills Oblique Angle Cutting, as shown inAtlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Amplifier Tube Glass Vacuum Tube (833C)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm) for the financial support.

REFERENCES

Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yard panel]

Brown, E. M; Thompson, C .J.,Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, M.M.(1994),Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Mode Sc/APC Simplex Fiber Optic Adapter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. Analytical Biochemistry212,436-445[L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm]

Dalev, P.G. and Simeonova, L.S.(1992),Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory. Biotechnology letters 14,6, 531-534[Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)]

Heidemann, E.(1997),Masturbators Adult Sex Toys Japanese Love Doll.Leder 48,(5),99-104[Spanish Stone Coated Steel Roof Tile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Newest Adjustable Decline Bench Flat Weight Bench for Wholesales]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Diameter Aluminum Pipe 3004]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12# X 14# Electric Galvanized Double Strands Barbed Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel CNC Turning Machining Processing Stainless Steel CNC Turning Machining Processing Engineering Mechanical Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

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12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Powder Spray Booth for Metal Furniture, Part I.Small Tin Box for Condoms. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Whitaker,J.R.(1963),Rubber Soles Vulcanizing Machine/ Press Machine. Analytical Chemistry35(12), 1950-1953[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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