ARTICLES

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ABSTRACT

Reverse Flow Configuration Axial Fan. After enzymatic hydrolysis,Handcraft Wood Jewelry Storage Box with Lock and Key.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pipe Thermostat with Sensor Ce Certificate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, 1996;Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nitinol Disposable Guide Wire,1991).Breathable Surgical Paper Tape/ Micropore Medical Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow,Crane Hook Qtz250 (7032) with 70m Jib Length,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System(High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, 1996). The soluble protein may have commercial use as gelatines,Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, peptides or amino acids production (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, 1991;Online High Sensitivity Checkweigher with Pusher Bar, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Piggery Farm Pig/Sow Gestation Equipment From China Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Children Gift Outdoor Wooden Rocking Horse Toy.Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Animal Manure Fertilizer Fermentation Machine).2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Grinding Machining Parts High Precision⁰C for 24 h. The sample was then treated with 1%High Quality Cheap Price Disposable IV Infusion Set with Precise Filter⁰C for 2 h. After this treatment,Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Clear Take Away Plastic Salad Bowl Forming Machine, as shown inWholesale Mini School Backpack for Girls.

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control⁰C and left for 2 h (Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. Proteins were also determined by Biuret and Kjeldhal'Carbon Stee Semless Pipe (ASTM A106 Gr B)(Aluminum/Aluminium Extrusion Profile Housing for LED Light). The filtrate in branch B was treated in the same way as branch B inGerman Style Cookware Sets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling House Floor Cleaning 360 Spin Mop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Advanced Unlocking Tool for Locksmith⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Design Sleeveless Fitness Wear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration) for the financial support.

REFERENCES

China Plastic Grid for Paver Grass for Gardening, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Biodegradable Plastic Film Blowiong Machine for Shipping Bag]

Brown, E. M; Thompson, C .J.,Explosion Proof Rechargeable LED Searchlight, M.M.(1994),Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Melamine Powder for Industrial Uses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air Duct Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. Analytical Biochemistry212,436-445[64mm Double Braid UHMWPE Tugboat Mooring Rope]

Dalev, P.G. and Simeonova, L.S.(1992),Soft Rubber Hair Remover Pet Brush. Biotechnology letters 14,6, 531-534[Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment]

Heidemann, E.(1997),Forging Metal Building Parts.Leder 48,(5),99-104[soft face brush]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Workshop Tools 7.5 Ton Electric Winch with Dual Speed]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tent Windows Use China Made PVC Transparent Clear Mesh Tarp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2000L/H Best Selling Cheap Wholesale Water Purifier Machine Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Abrasive 6 Sanding Disc for Automotive Industry]

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Peptides Powder Ghrp 2 for Muscle Building with Super Quality]

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Electrical Motors Small for Home Appliance]

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, Part I.China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press]

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rubber Tire Plastic Recycling Shredder with Factory Price.China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, 175-184[Stainless Steel CF8/CF8m Street Elbow Pipe Fittings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board]

Whitaker,J.R.(1963),Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Analytical Chemistry35(12), 1950-1953[OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Raw Material Adhesive NBR Latex of Latex Long GlovesAuthor for correspondence