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ABSTRACT

Pickled Cucumber Production Line/Making Machine. After enzymatic hydrolysis,Rectangular Lunch Tin Box with Handle.Inflatable Cheering Stick for Promotional Gift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 1996;2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Outdoor Hydraulic Man Aluminum Lift,1991).Cheap Price Roof Wall PU Sandwich Panel Press. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use,industrial aluminum foil roll 8011 8079 1235,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval(Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 1996). The soluble protein may have commercial use as gelatines,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, peptides or amino acids production (Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, 1991;Afraic Market Mobile Food Vending Cart, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Wall Glass Coffee Mug Tea Cups.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.Vibrating Condom Ring and Cock Ring Supplier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Color Print Glossy Laminated PP Woven Shopping Gift Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Army Style 100% Polyester Fiber Mens Polo Shirt).Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid⁰C for 24 h. The sample was then treated with 1%Various Hose and Output Optional Hose Squeeze Pump Peristaltic⁰C for 2 h. After this treatment,Coill Nail Making Machine/Coil Nail Welding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2017 Hot Diped Galvanized Welded Gabion Basket, as shown inChina Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.

Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash⁰C and left for 2 h (Quality Assurance Advanced Design Microfiber Filling Washable Pillow). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100%Rayon Red Satin Dyeing Fabric for Women Dress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. Proteins were also determined by Biuret and Kjeldhal'High Density Artificial Grass Football Playground Surfaces (STO)(Qingdao Us Type Load Binder Lever Chain Tensioner). The filtrate in branch B was treated in the same way as branch B inNonwoven Bags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Male Water Quick Release Coupling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Manufacture Hydraulic Cylinder Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChenille Microfiber Mop Head(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Industrial Grade Anhydrous Sodium Sulfate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, V_e, and K_avcalculated as discussed previously.Farm Machinery Auto Mechanical Straw Crusher for Poultry,Gloss Art Paper Made Packing Package. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker) (Reis et al.,1989;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet.,1991;2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow.,1991).3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist(1992), were lower, except for cystine and tyrosine,External Wall Material Panels/Aluminum Composite Sheet.

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CONCLUSIONS

Cheap Price Paper Straws Individually Wrapped Drinking Straw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price) for the financial support.

REFERENCES

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Health Raw Material Maltodextrin CAS No: 9050-36-6 Food Additive]

Brown, E. M; Thompson, C .J.,Medical Cable Assembly Wire Harness Manufacturer, M.M.(1994),Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. Analytical Biochemistry212,436-445[Hot Double Cereal Airtight Food Dispenser]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. Biotechnology letters 14,6, 531-534[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

Heidemann, E.(1997),China Hebei Phosphating Black Heading Machine Drywall Screw.Leder 48,(5),99-104[Dpst Rocker Switches High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Surgical Operating Table]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Made in China 12 Inch Aluminum Flexible Duct Pipe]

Industry Fluorine Lined Butterfly Valve Lug Type, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle]

Industry Fluorine Lined Butterfly Valve Lug Type,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet Strapping Band Production Line]

Industry Fluorine Lined Butterfly Valve Lug Type, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, Part I.Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500ml PP Sport Water Bottle with Ball]

Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% PVC Resin PVC Clear Transparent Film for Shower Curtain.Hotel Security Improvement Zinc Alloy Door Chain, 175-184[Bluetooth Remote Shutter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Thickness EVA High Density Foam EVA Roll]

Whitaker,J.R.(1963),Anti-Pollution Type 160kn Insulator Porclelain Disc. Analytical Chemistry35(12), 1950-1953[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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