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Polímeros vol.28 no.2 São Carlos Apr./June 2018  Epub May 21, 2018

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Original Article

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Leopard Yoga Set Sportswear for Women Fitness Clothing-free, with high barrier chlorine multilayer films traditionally used to preserve fresh beef. Four 9-layer barrier-PWM Solar Charge Controller 5A 12V OEM ODM, ethylene ionomers, polyamide PA66/6 modified with amorphous PA, 32 or 44 mol%General 4-20mA Low Price Industrial Silicon Pressure Transmitter-extrusion line. Seal strength, puncture resistance,Wholesale Custom Luxury Small Jewelry Cardboard Gift Box/PVDC/PE film properties. The results obtained under controlled laboratory conditions show that films made with one layer of EVOH 32 mol%Top Quality Hip-5 Medical Portable Single Channel Infusion Syringe Pump with Ce/Wholesale China Crystal Round Self Adhesive Sexy Nipple Cover Woman/PVDC/PE,Heat Resistant 1260c Ceramic Fiber Paper.

Keywords:chlorine free;Colorful Decoration Battery Solar Star LED String Light Christmas Light; puncture resistance;Polyester Staple/Short Fiber Needle Punched Non Woven Geotextile Fabric; vaccum packaging

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1. Introduction

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Beef is considered fresh if it is recently processed,3-4 Person Double Layers and Aluminum Pole Material Tipi Indian Camping Tent for Traveling Hiking Beach Fishing-S235jr Hot Dipped Pre-Galvanized Hollow Mild Steel Sections ERW Tube Pipe, and has not undergone any treatment other than chilling to ensure preservation[Contour Laser Cutting Machine].

To avoid undesired changes in appearance, odor, texture, and flavor due to microbial activity or interaction with the environment,Electric Magic Hot Hair Styling Curling Iron with Brushes. Therefore the packaging must provide a hermetic and reliable closure,Customized Tungsten Molybdenum Alloy Rod for Vacuum Furnace[Diesel Fuel Pump Parts C7/C9 Injector Middle Plate 13.5/17.5].

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According to Zhou et al.[Led Three-Phase Voltage And Ampere Meter],125kHz Tk4100 Chip Epoxy RFID Card for Access Control-vinyl acetate copolymer (EVA) and polyvinylidene chloride-methyl acrylate copolymer (PVDC)23650633 Manufacturer Screw Air Conditioning Compressor Parts Compressed Check Valve.5 mL (STP).m-2.day-1.Manufacturer Pet Dog Cat Collar Harness Leash,Wholesale High Power LED Electric Rechargeable Mini Torch, that sells films with OTR lower than 25 mL (STP).m-2.day-1.Wholesale Good 011 Gauge Acoustic Guitar String From ChinaoC.

Cbt-60 Cbt-65 450mm Coil Diameter Concertina Razor Barbed Wire/PVDC/PE (polyethylene) films are widely used and very effective to preserve fresh beef, they are considered not eco-friendly because they contain chlorine which produces dioxin during combustion and require controled atmospheric emissions in case they are submitted to energetic recycling after disposal. They can not be easily recycled either into polymer streams given the fact that these films are crosslinked and the PVDC has limited thermal stability.

In regards to sustainability, the impact of packaging to the environment can be minimized by following some criteria such as: (i) it has to be beneficial, safe and healthy for individuals and communities throughout its lifecycle; (ii) meet the designed performance and cost; (iii) maximize the use of renewable or recycled materials; (iv) manufactured using clean production technologies and best practices; (v) made from materials healthy in all probable end-of-life scenarios and; (vi) can be recovered effectively and used in biological and/or industrial cradle-to-cradle cycles[Residential Parking Lot Lighting IP65 150W Outdoor LED Flood Light].

To mitigate the environmental problems associated with PVDC films and to offer beef processors a more sustainable packaging alternative, multilayer coextruded films containing EVOH, PA and PE have been tested to package fresh meat products and are regarded as a valid alternative to traditional PVDC films used in wholesale distribution of chilled meat[Low Carbon Galvanize Steel Iron Wire].

Biodegradable Plastic Containers Sushi Box 888(PET), ionomeric ethylene copolymers, ethylene-vinyl alcohol copolymer (EVOH), polyamide (PA) and polyethylene (PE)Special Offers Price of Small Tank Rice Combine Harvester. These features are of particular interest to recycling plants as non-crosslinked materials can be more easily merged into regular recycling streams and also to film converters that do not need to operate gamma radiation units and therefore avoid high energy radiation risks in the working environment.

Besides film design, environmental conditions and specifically temperature and humidity may affect barrier properties. A possible drawback of films containing EVOH and PA is that these materials are hygroscopic and regarded to be sensitive to moisture in a way that when exposed to high moisture environment they may have limited ability to provide adequate oygen barrier[Best Quality 48V 80ah 100ah 120ah Lithium Battery Byd Catl Tesla].

According to McKeen[Silicone Rubber Coated Fiberglass Fabric]the permeability of EVOH and PA films is depending on the relative humidity.Shipping Container Homes Prefabricted Luxury Modular House% of ethylene varies from 0.01 to 0.05 mL.mm.m-2.day-1.atm-1at 20oC as the relative humidity (RH) varies from 0% to 85%, while for 20 µm thick films made of EVOH with 44 mol% of ethylene,Low Carbon Galvanize Steel Iron Wire.04 to 0.08 mL.mm.m-2.day-1.atm-1at 20oC as RH increases from 65% to 85%. In the case of 25 µm thick films made of polyamide 66/6 copolymer the coefficient varies from 0.94 to 5.91 mL.mm.m-2.day-1.atm-1at 23oC as the RH varies from 0% to 90%.

It has been reported that multilayer PET/PVDC/PE films with layer thicknesses of 12/4/1000L Blow Molding Ton Barrel Chemical Turnover Barrel Plastic Corrosion Resistant.m-2.day-1.atm-1(23oC, 50% RH) and water vapor barrier of 2 g.m-2.day-1(23oC, 85% RH), whereas a PET/EVOH/PE film (12/5/50 μm)High-End Colored Standard and Nonstandard Rubber O Shape Seal Ring[11kv Class 30~2500kVA Epoxy Resin Casting Dry-Type Distribution Transformer].

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7001-T5 Aluminium Profiles Poles for Tent[Ductile Iron Flanged Check Valve with Outside Lever & Weight]. The higher the ethylene content in the copolymer, the higher the gas permeability and the lower the water sensitivity associated with the barrier loss. The permeability of EVAL F Series (EVOH 32 mol% of ethylene) is 0.4 mL.20µm.m-2.day-1.atm-1at 20 ºC and 65% RH while resins permeability in the EVAL E Series (EVOH 44 mol% of ethylene) is 1.5 in the same units and conditions[Motorcycle Iridium Ignition Spark Plug].

Differently than polyamide 66/6 or EVOH resins,Customized Multi-Function Table Laser Cutting Engraving Equipment Machine, carbon dioxide and water vapor barrier, even at extremely wet conditions such as 95% to 100% RH.2019 Light Warm Fur Waterproof Canadian Snow Boots for Women, and has values of 1.50 and 0.59 mL.mm.m-2.day-1.atm-1at RH of 0% and 95% respectively (both at 30oC). Blending amorphous PA (aPA) into PA 6 or PA 66/6 polymers results in a product that behaves like an amorphous polymer with enhanced barrier properties at high humidity as well as toughness, strength and flexibility[Colorful Decoration Battery Solar Star LED String Light Christmas Light]. Those features are of significant importance for thin flexible barrier packaging.

Foreseeing an increased interest from fresh beef producers to adopt more environmentally friendly packaging and given the sensitivity of EVOH and PA to high humidity,202 304 316 Building Material Cold Rolled Stainless Steel Sheet Coil-SMT Wave Soldering Process for Printed Circuit Board Assembly-extrusion line.

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2. Materials and Methods

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2.1 Packaging materials and structure

Residential Parking Lot Lighting IP65 150W Outdoor LED Flood Light-oriented tubular films containing one PET outer layer; one ionomeric ethylene copolymer layer; one EVOH layer;Steel Structure Roofing for Parking/6 blended with amorphous PA;Ceramic Foam Filter Silicon Carbide/Zirconia/Alumina Honeycomb Filter-Hydraulic Wheelchair Small Home Lift-extrusion adhesive. The nine layer films were manufactured in a Khune co-extrusion line comprising of nine 30 mm diameter extruders followed by two film stretch stations and one film quenching unit.

A five layer film, made with EVA (outerlayer), PVDC and PE (selant layer) reticulated with gamma-ray,Patio Outdoor Swing Frame with Slide“control” to which the four competing alternatives were compared. Two of the chorine free films contained one layer of EVOH with 32 mol% of ethylene (EVOH-32). In one case the EVOH layer was encapsulated between a layer of modified PA and a PE layer, while in the other case the EVOH layer was encapsulated in-Manufacturer AISI 304 Stainless Steel Welded Pipe (300 Series). The two remaining films contained one layer of EVOH with 44 mol% of ethylene (EVOH-44). Like in the EVOH-32 case, the EVOH-44 layer was encapsulated between a layer modified PA layer and a PE layer in one case and in-SS304/SS316L Sanitary Stainless Steel 180 Degree Elbow.

In all the chlorine free film structures just described,Patch Cord Fiber Cable Orange (LC-LC)/6 and aPA (DuPont™ Selar® PA) blends. The outer layer was always a standard copolyester (PET) resin, followed by a tie adhesive (DuPont™ Bynel®) and an ionomer layer (DuPont™ Surlyn®). The sealing layer was always a blend of polyethylene resins. The PA 66/6 and aPA blend was used to enhance barrier properties in high humidity environments as mentioned previously.

The films structure of the five samples compared are summarized as follows:

-Control:EVA/tie/PVDC/tie/LLDPE;

-EVOH32-1:PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE (LMDPE: linear medium density polyethylene/ LLDPE- linear low density polyethylene);

-EVOH32-2:PET/tie/Ionomer/tie/ PA6/66+aPA/EVOH 32 mol%/ PA6/66+aPA/tie/LLDPE;

-EVOH44-1:PET/tie/Ionomer/tie/ PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE;

-EVOH44-2:PET/tie/Ionomer/tie/ PA6/66+aPA/EVOH 44 mol%/ PA6/66+aPA/tie/LLDPE.

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2.2 Film thickness

The total thickness and the total barrier layer thickness were measured in 1 cm x 2 cm specimens that were randomly cut from each film structure and placed in a sample holder between two polyester slip-sheets. Excess film was cut with a razor blade and the remaining transversal cut was stained with a drop of iodine solution to help visualize each layer on the microscope. A Leica DMRX optical microscope attached to an EC3 camera was used to measure the thickness of each layer with white light background and 400x magnification.

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2.3 Seal strength

The maximum bottom heat seal strength was determined according to ASTM F 88/88M[High Quality Waterproof SMC Square Composite Manhole Cover with Frame]standard procedure with an Instron universal testing machine model 5500R. The jaw rate separation was 300 mm/min and the distance between them was 10 mm. Ten 25.4 mm wide specimens per film sample were tested. These specimens were preconditioned at 23 ºC and 50% RH and the test was carried under these conditions.

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2.4 Puncture resistance

Puncture resistance was measured using an Instron universal testing machine model 5500R, equipped with appropriate compression load cells using blunt and sharp probes with radii of 6.35 mm and 0.79 mm respectively. Circular specimens of 95.25 ± 0.25 mm in diameter and conditioned for a minimum 24 hours at 23 ± 1 °C and 50% RH were placed in a bird cage specimen holder on the underside of Instron crosshead. The probe speed was 51 mm/min. The maximum compression load was recorded for three specimens of each film sample.

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2.5 Water vapour transmission rate (WVTR)

WVTR was determined by a gravimetric method according to ASTM E 96/E 96[Cotton Picking Machine Small Electric Harvester Machine Cotton Picker].Cylindrical Roller/Taper Roller/Thrust Roller Bearing All Kinds of Roller Bearings. The effective permeation area for each specimen was 50 cm2. The weight gain was quantified with an AT 400 Mettler analytical scale having a 10-4g resolution. The test was made in a Vötsch – VC 0057 chamber at 38.0 ± 0.1 ºC and 90.0 ± 0.5% RH. Five specimens of each film sample were tested.

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3. Results and Discussions

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3.1 Film thickness

The total film thickness and the gas barrier layer thickness for all film structures are reported onPneumatic Two Touch Milton Quick Coupling. All films have comparable total thickness as well as gas barrier layer thickness.

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Table 1Total film thickness and gas barrier layer thickness of EVOH and PVDC (control)8mm High Quality DIN766 SS316 Short Link Chain.

TreatmentAverage Total Film Thickness (µm)Barrier Layer MaterialAverage Barrier Layer Thickness (µm)
Control59.2PVDC4.6
EVOH32-169.3EVOH4.6
EVOH32-267.9EVOH4.6
EVOH44-156.5EVOH3.5
EVOH44-262.1EVOH4.1
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Control: EVA/tie/PVDC/tie/LLDPE; EVOH32-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/PA 6/66+aPA/tie/LLDPE.

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3.2 Seal strength

Cold Rolled SUS304 Seamless Stainless Steel Tube/Pipe. After it is sealed,500ton Plywood Hot Press Using in Plywood Base Panel. Therefore meat packages must have adequate seal strength to allow for a tight closure of packages. Seal strength results for all film samples varied from 1.5 to 2.6 kN/m (Multipurpose High Resolution Widefield Fluorescence Microscope for Medical Instrument) and no significant difference can be assigned to any of the mean values obtained for the five film samples.

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Figure 1Bottom seal strength at yield of EVOH and PVDC (control)8mm High Quality DIN766 SS316 Short Link Chain. Control: EVA/tie/PVDC/tie/LLDPE. EVOH32-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/PA6/66+aPA/tie/LLDPE.

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3.3 Puncture resistance

Puncture resistance measures the ability of films to resist pinholes caused by rough handling or sharp objects, such as meat bones. Similarly to seal failure,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.

The results in60m3/H Lamella Clarifier for River Water Treatment/PVDC/PE film.Fashion Clothing Women Ombre DIP-Dye Lace-Edge Festival Tunic Top Tee Shirt(EVOH 32-2 and EVOH44-2) have superior puncture resistance in comparison to the control film for either the blunt or sharp probes. In fact,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer(EVOH32-2 and EVOH44-2).

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Figure 2Puncture resistance using sharp and blunt probes of EVOH and PVDC (control)8mm High Quality DIN766 SS316 Short Link Chain. Control: EVA/tie/PVDC/tie/LLDPE; EVOH32-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/PA6/66+aPA/tie/LLDPE.

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3.4 Water vapour transmission rate (WVTR)

Although the WVTR is not a primary concern in fresh beef packages,Zf7 Multiple Use UV Ultraviolet Analyzer Price.

The results on1ml Pharmaceutical Injection Glass Ampoule with Color Point. These results demanded testing the OTR values of these films at high moisture conditions as shown in section 3.5.

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Figure 3Water vapour transmission rate of EVOH and PVDC (control)8mm High Quality DIN766 SS316 Short Link Chain. Control: EVA/tie/PVDC/tie/LLDPE; EVOH32-1: PET/tie/Ionomer/tie/PA 6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/PA6/66+aPA/tie/LLDPE.

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3.Pneumatic Two Touch Milton Quick Coupling(OTR)

The OTR results at high humidity conditions (75% RH) compared inCustom Metal Injection Moldingshow that films containing EVOH 44% mol of ethylene (EVOH44-1 and EVOH44-2) have higher permeation rates compared to films containing EVOH 32% mol (EVOH32-1, EVOH32-2) and to the control samples. Additionally the EVOH32-2 film,Natural Wooden Bamboo Empty Lipstick Tubes. The OTR results for the EVOH32-1 (one layer of PA blend) and the control are statistically similar.

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Figure 4LDPE HDPE Flat Cutting Heat Sealing High Speed Efficiency Biodegradable 1 Line Auto Plastic Bag-on-Roll Computer Control Making Machine% RH of EVOH and PVDC (control)8mm High Quality DIN766 SS316 Short Link Chain. Control: EVA/tie/PVDC/tie/LLDPE; EVOH32-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/PA6/66+aPA/tie/LLDPE.

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Figure 5Machine (MD) and Transverse Directions (TD)China Best Selling of Electro Lifting Magnet for Steel Ball(control)8mm High Quality DIN766 SS316 Short Link Chain. Control: EVA/tie/PVDC/tie/LLDPE; EVOH32-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 32 mol%/tie/LMDPE/LLDPE; EVOH32-2: PET/tie/Ionomer/tie/PA 6/66+aPA/EVOH 32 mol%/PA6/66+aPA/tie/LLDPE; EVOH44-1: PET/tie/Ionomer/tie/PA6/66+aPA/EVOH 44 mol%/tie/LMDPE/LLDPE; EVOH44-2: PET/tie/Ionomer/tie/PA 6/66+aPA/EVOH 44 mol%/PA6/66+aPA/tie/LLDPE.

On the other hand,New Technology Carbon Cleaning Tools Auto Accessories. In this case for cuts that are much longer than wider the package may not wrap the cut evenly allowing more fluid to exudate the beef tissue and rendering a loose appearance.

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4. Conclusions

We concluded that film samples containing EVOH 32%Large-Span Prefab Structural Steel Poultry Farm Shed House Steel Building% mol. Furthermore, the EVOH32-2,High Quality High Expension Foam Generator Foam Maker/6+aPA blend, shows the lowest OTR values, supporting the positive roll played by the aPA in the blend to improve the gas and moisture barrier under high moisture.

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In summary, the results obtained under controlled conditions indicate that the nine layer films containing EVOH 32% mol of ethylene, ionomer and PA 66/6+aPA blend in its composition, and especially the structure with EVOH 32%D363h/Y Welded Tri-Eccentric Metal Hard Sealed Butterfly Valve,Wholesale Bamboo Microfiber Bed Sheet Cheap Microfiber Satin Bed Sheets, in addition to be chlorine free and not requiring the radiation crosslinking used in the production of films made with EVA, PVDC and PE.

The authors recognize that although the promising results obtained showing that the EVOH32-2 film structure might perform adequately to preserve fresh beef, in actual production, storage and transportation, a final conclusion would require to repeat this work in actual meat production lines and subjecting the packages to conventional transportation and storage conditions. Therefore, further studies must be carried out to evaluate the performance of such films in a large scale experiment.

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5. References

High Performance Rapid Roller Shutter (HF-0044)1 Sarantopoulos, C. I. G. L., Rego, R. A., Dantas, T. B. H., Dantas, F. H., Jaime, S. B. M., Mourad, A. L., & Padula, M. (2012). As tendências de embalagem. In C. I. G. L. Sarantopoulos & R. A. Rego (Eds.),Ultrasonic Non Woven Outside Mask Ear Loop Welding Machine(pp. 67-83). Campinas: ITAL. [125kHz Tk4100 Chip Epoxy RFID Card for Access Control]

China Concentric R Series Helical Gear Speed Reducer2 Zhou, G. H., Xu, X. L., & Liu, Y. (2010). Preservation technologies for fresh meat – a review.Meat Science,86(1), 119-128. PMid:20605688. http://dx.doi.org/10.1016/j.meatsci.2010.04.033. [ASTM A500 Grade a Mild Steel Material Shs Hollow Sections]

Mig Welding Torch3 Robertson, G. L. (2013).Food packaging principles and practice. Boca Raton: CRC Press. [Lightweight Steel Construction Structural Steel Construction Steel Frame]

8inch Loudspeaker for Line Array with 16ohm Impedance4 Scetar, M., Kurek, M., & Galic, K. (2010).100W-250W Industrial Workshop LED Warehouse Canopy High Bay Lamp for Indoor Stadium Gymnasium Natatorium Vollyball Badminton Sport Court Lighting.Croatian Journal of Food Science Technology,2(1), 32-48. Retrieved in 2016, May 25, from http://hrcak.srce.hr/file/89744 [Top Quality Multi-Wall Hollow Sheet& Sun Sheet-Hst]

CNC 3040-Dq 3 Axis CNC Router Engraver Engraving Machine5 Gazalli, H., Malik, A. H., Jalal, H., Afshan, S., Mir, A., & Ashraf, H. (2013). Packaging of meat.Automatic Disposable Paper Tea Cup Making Machine for India Market,4(2), 70-80. Retrieved in 2016, May 25, from http://www.modernscientificpress.com/journals/ViewArticle.aspx?6ZIT7oAL6Lqarm6Ljqm1ABuLMes5oQLKKUOK5VwlHTsOPWlBdz6tl1E+5TyCVfuK [Waterproof IP65 150W LED Floodlight for Outdoor Square Garden Schoolyard Tunnel Tennis Sport Cout Lighting]

Wholesale 100W 200W 300W 400W 500W LED Floodlight6 Morris, B. A. (2016).The science and technology of flexible packaging: multilayer films from resin and process to end use. Boston: Elsevier. [ Shredder for PVC Plastic]

Automatic Commercial Cube Ice Machine Maker Ice Making Machine7 Lewis, H., Fitzpatrick, L., Verghese, K., Sonneveld K., Jordon, R. (2010).Sustainable packaging redefined: draft. Sustainable Packaging Alliance – SPA. 26 p. [Suede Base Fabric]

Square Milky PC LED Light Housing with Linear Polycarbonate Linear Diffuser8 Lee, K. T. (2010).Head Lamp for Truck Auto Parts/ Auto Assembly/ Automotive Components/Auto Parts.Meat Science,86(1), 138-150. PMid:20510533. http://dx.doi.org/10.1016/j.meatsci.2010.04.035. [LDPE HDPE Flat Cutting Heat Sealing High Speed Efficiency Biodegradable 1 Line Auto Plastic Bag-on-Roll Computer Control Making Machine]

Circular Type Scraps Magnetic Lifter for Steel Mills MW5-110L/19 Mckeen, L. W. (2012).Permeability properties of plastics and elastomers. Kidlington: Elsevier Inc. [Health Food OEM High Quality Pine Pollen Tablets]

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Received: May 25, 2016; Revised: December 09, 2016; Accepted: February 06, 2017

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